目的 对匙萼金丝桃(
Hypericum uralum)化学成分进行分离鉴定;对部分成分的镇痛与
β-羟高铁血红素形成抑制活性进行测试。
方法 利用硅胶、Sephadex LH-20等材料柱色谱和现代波谱技术对匙萼金丝桃体积分数95%乙醇提取物乙酸乙酯部分化学成分进行分离和结构鉴定;采用小鼠乙酸扭体法、热水甩尾法和
β-羟高铁血红素形成抑制实验对部分单体化合物进行生物活性测试。
结果 从匙萼金丝桃体积分数95%乙醇提取物乙酸乙酯部位中分离得到13个化合物,分别鉴定为(-)-圣草素(1),3,4,5-三羟基
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酮(2),1,7-二羟基
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酮(3),4-羟基-2,3-二甲氧基
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酮(4),toxyloxanthone B(5),1,3,6,7-四羟基
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酮(6),2,3-二甲氧基
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酮(7),1,5,6-三羟基-3-甲氧基
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酮(8),hyperielliptone HD(9),3,3′,4,4′-四羟基联苯(10),莽草酸(11),槲皮素-3-
O-(4″-甲氧基)-
α-L-鼠李糖(12)和原花青素A-2(13)。镇痛活性测试结果表明,化合物3和12具有一定的外周镇痛活性;同时,化合物1表现出较强的
β-羟高铁血红素形成抑制活性。
结论 化合物1~13均为首次从该植物中分离得到;匙萼金丝桃镇痛与抗疟活性具有相应物质基础。
Abstract
OBJECTIVE To study the chemical constituents of Hypericum uralum were isolated and identified and test the analgesic activity and β-hematin formation inhibitory activity of some selected compounds. METHODS The compounds were isolated and purified by column chromatography padded with the separating material including silica gel and Sephadex LH-20, and their structures were elucidated based on the analyses of modern spectrum technology. Analgesic and antimalarial activities of selected compounds from H. uralum were evaluated by acetic acid-induced writhing, hot water tail-flick test in mice, and β-hematin formation inhibition method, respectively. RESULTS Thirteen compounds were isolated from ethyl acetate portion of 95% ethanol extract of H. uralum and identified as (-)-eriodictyol (1), 3,4,5-trihydroxyxanthone (2), 1,7-dihydroxyxanthone (3), 4-hydroxy-2,3-dimethoxyxanthone (4), toxyloxanthone B (5), 1,3,6,7-tetrahydroxyxanthone (6), 2,3-dimethoxyxanthone (7), 1,5,6-trihydroxy-3-methoxyxanthone (8), hyperielliptone HD (9), 3,3′,4,4′-tetrahydroxybiphenyl (10), shikimic acid (11), quercetin-3-O-(4″-methoxy)-α-L-rahmnopyranosyl (12), and proanthocyanidin A-2 (13). The analgesic activity test indicated that compounds 3 and 12 had certain peripheral analgesic activity, while compound 1 exhibited strong β-hematin formation inhibitory activity. CONCLUSION All the compounds are obtained from this plant for the first time.The analgesic and antimalarial activities of H. uralum have corresponding material basis.
关键词
匙萼金丝桃 /
(-)-圣草素 /
1 /
7-二羟基![]()
酮 /
镇痛活性 /
β-羟高铁血红素形成抑制活性 /
抗疟活性
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Key words
Hypericum uralum /
(-)-eriodictyol /
1,7-dihydroxyxanthone /
analgesic activity /
β-hematin formation inhibitory activity /
antimalarial activity
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中图分类号：
R965
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脚注
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基金
国家自然科学基金项目资助(81460532,81641187)
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